CYTOSOL

TECHNICAL PRODUCT BULLETIN #SW-19
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: JANUARY 30, 1997
"CYTOSOL"
LAST COMMUNICATION WITH MANUFACTURER: 3/03/2016
LAST ATTEMPT: 7/29/2021

I. NAME, BRAND, OR TRADEMARK
CYTOSOL Type of Product: Surface Washing Agent

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER
CytoCulture International, Inc. 249 Tewksbury Avenue Point Richmond, CA 94801-3829 Phone: (510) 233-0102 Fax: (510) 233-3777 Emergency Mobile: (510) 233-0102 E-mail First Response: rvwedel@gmail.com E-mail: cytoculture@gmail.com Website: www.cytosolbiosolvent.com Website: www.cytoculture.com (Dr. Randall von Wedel)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
CytoCulture International, Inc. 249 Tewksbury Avenue Point Richmond, CA 94801-3829 Phone: (510) 233-0102 Fax: (510) 233-3777 Emergency Mobile: (510) 233-0102 E-mail First Response: rvwedel@gmail.com E-mail: cytoculture@gmail.com Website: www.cytosolbiosolvent.com Website: www.cytoculture.com (Dr. Randall von Wedel) Foss Environmental, Inc. 7440 West Marginal Seattle, WA 98108-4141 Phone: (206) 768-1450 Fax: (206) 767-3460 (Mr. Larry Pintler) Advanced Cleanup Tech. Inc 20928 Lamberton Ave. Carson, CA 90810 Phone: (800) 334-2284 Fax: (310) 763-9076 (Mr. Walt Dorn)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS

CytoCulture International, Inc.
249 Tewksbury Avenue
Point Richmond, CA 94801-3829
Phone: (510) 233-0102
Fax: (510) 233-3777
Emergency Mobile: (510) 233-0102
E-mail First Response: rvwedel@gmail.com
E-mail: cytoculture@gmail.com
Website: www.cytosolbiosolvent.com
Website: www.cytoculture.com
(Dr. Randall von Wedel)

Foss Environmental, Inc.
7440 West Marginal
Seattle, WA 98108-4141
Phone: (206) 768-1450
Fax: (206) 767-3460
(Mr. Larry Pintler)

Advanced Cleanup Tech. Inc
20928 Lamberton Ave.
Carson, CA 90810
Phone: (800) 334-2284
Fax: (310) 763-9076
(Mr. Walt Dorn)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable. However, keep the product away from heat and avoid contact with strong oxidizing agents. Ensure proper disposal of product-saturated absorbents, rags, and combustible materials to avoid the possibility of spontaneous combustion. 2. Ventilation: Product is not volatile. However, in the event of aerosol inhalation, immediately move victim to fresh air. If victim has stopped breathing, give artificial respiration, preferably by mouth to mouth. Get medical attention immediately. 3. Skin and eye contact; protective clothing; treatment in case of contact: The CYTOSOL contains no volatile hydrocarbons or petroleum constituents. However, as a precautionary measure, wear gloves and safety glasses meeting the specifications of ANSI Standard Z87.1. Avoid breathing aerosols. Avoid prolonged contact with skin. 4. Storage: a. Maximum storage temperature: 110ºF b. Minimum storage temperature: 39ºF c. Optimum storage temperature: 55ºF d. Temperatures of phase separations and chemical changes: Avoid freezing. At temperatures below the cloud point (43ºF), the product may become cloudy, but will return to normal upon warming, with no effect on performance. Store product in airtight containers, if possible, without excessive exposure to moisture.

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Non-flammable. However, keep the product away from heat and avoid contact with strong oxidizing agents. Ensure proper disposal of product-saturated absorbents, rags, and combustible materials to avoid the possibility of spontaneous combustion.
2. Ventilation:
Product is not volatile. However, in the event of aerosol inhalation, immediately move victim to fresh air. If victim has stopped breathing, give artificial respiration, preferably by mouth to mouth. Get medical attention immediately.
3. Skin and eye contact; protective clothing; treatment in case of contact:
The CYTOSOL contains no volatile hydrocarbons or petroleum constituents. However, as a precautionary measure, wear gloves and safety glasses meeting the specifications of ANSI Standard Z87.1. Avoid breathing aerosols. Avoid prolonged contact with skin.
4. Storage:
a. Maximum storage temperature: 110ºF
b. Minimum storage temperature: 39ºF
c. Optimum storage temperature: 55ºF
d. Temperatures of phase separations and chemical changes:
Avoid freezing. At temperatures below the cloud point (43ºF), the product may become cloudy, but will return to normal upon warming, with no effect on performance. Store product in airtight containers, if possible, without excessive exposure to moisture.

V. SHELF LIFE:
Closed container: 10 years in a dry environment. Open container: 1 year in a warm, humid environment. The product does not deteriorate appreciably over time, but will grow bacteria if water condensation accumulates in the container.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: The CYTOSOL is applied to oiled shorelines to extract and recover weathered petroleum by flotation with passive water deluges from header pipes or manual spraying. Remaining residual hydrocarbons are biodegraded, either passively by intrinsic bioremediation, or aggressively by enhancing the process with controlled amounts of nutrients and/or acclimated cultures of bacteria cultured from the site, when approved by local, state and federal agencies. The CYTOSOL Process is most suitable for the treatment of heavily oiled shorelines that do not respond well to conventional treatments, or that are considered too sensitive for mechanical/pressure wash strategies. Prior to the application of CYTOSOL, collection booms, oil skimmers, sorbent pads, or other appropriate containment and collection mechanisms must be deployed and operational. 2. Concentration/Application Rate: CYTOSOL may be applied with a variety of spraying or washing equipment, depending upon the scale and type of shoreline to be cleaned. The product is to be used only neat and undiluted, for direct application to spilled oil. For large beach areas, CYTOSOL can be sprayed from water trucks or work boats equipped with pumps, hoses, and nozzles to deliver the product as an aerial spray. In smaller applications, CYTOSOL may be applied with hand sprayers or portable pumps to spray the product directly onto oiled surfaces. Dose rates will vary with the type and amount of petroleum spilled, the extent of weathering, and other site specific conditions, including temperature, porosity of shoreline, and residence time available to let the product contact the oil. In general, the ratio of applied CYTOSOL to crude oil is between 0.5:1 and 1:1. The quantity of CYTOSOL applied should be approximately equivalent to the quantity of petroleum accumulated on the shoreline, or as required to dissolve and remove weathered oil. After application, the product should be allowed to penetrate and dissolve the weathered petroleum for at least one hour, preferably longer. Cold weather applications will require more contact time before initiating recovery. In tidal areas, it is advisable to apply the CYTOSOL as the tide is ebbing (receding) to maximize contact time. Trapped oil may continue to be released for several days, requiring that containment devices be left in place. 3. Conditions for Use: The following shoreline types are appropriate for the use of CYTOSOL: Coarse sand beaches where petroleum has penetrated into sand; marsh areas and vegetated wetlands where oil has coated plants and become trapped; concrete bulkheads, rip rap and piers that may have trapped oil; oiled pilings; gravel or cobble shorelines and rocky shores, where oil has become trapped in pockets; and, public beaches, fisheries, hatcheries, river banks, and other sensitive or high impact sites. The CYTOSOL has been field tested successfully for removing oil from mussel beds and intertidal zones, pilings and concrete rip rap. The CYTOSOL also proved effective in facilitating the removal of oil from the banks and vegetation along an oiled creek.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
The CYTOSOL is applied to oiled shorelines to extract and recover weathered petroleum by flotation with passive water deluges from header pipes or manual spraying. Remaining residual hydrocarbons are biodegraded, either passively by intrinsic bioremediation, or aggressively by enhancing the process with controlled amounts of nutrients and/or acclimated cultures of bacteria cultured from the site, when approved by local, state and federal agencies. The CYTOSOL Process is most suitable for the treatment of heavily oiled shorelines that do not respond well to conventional treatments, or that are considered too sensitive for mechanical/pressure wash strategies. Prior to the application of CYTOSOL, collection booms, oil skimmers, sorbent pads, or other appropriate containment and collection mechanisms must be deployed and operational.
2. Concentration/Application Rate:
CYTOSOL may be applied with a variety of spraying or washing equipment, depending upon the scale and type of shoreline to be cleaned. The product is to be used only neat and undiluted, for direct application to spilled oil. For large beach areas, CYTOSOL can be sprayed from water trucks or work boats equipped with pumps, hoses, and nozzles to deliver the product as an aerial spray. In smaller applications, CYTOSOL may be applied with hand sprayers or portable pumps to spray the product directly onto oiled surfaces. Dose rates will vary with the type and amount of petroleum spilled, the extent of weathering, and other site specific conditions, including temperature, porosity of shoreline, and residence time available to let the product contact the oil. In general, the ratio of applied CYTOSOL to crude oil is between 0.5:1 and 1:1. The quantity of CYTOSOL applied should be approximately equivalent to the quantity of petroleum accumulated on the shoreline, or as required to dissolve and remove weathered oil. After application, the product should be allowed to penetrate and dissolve the weathered petroleum for at least one hour, preferably longer. Cold weather applications will require more contact time before initiating recovery. In tidal areas, it is advisable to apply the CYTOSOL as the tide is ebbing (receding) to maximize contact time. Trapped oil may continue to be released for several days, requiring that containment devices be left in place.
3. Conditions for Use:
The following shoreline types are appropriate for the use of CYTOSOL: Coarse sand beaches where petroleum has penetrated into sand; marsh areas and vegetated wetlands where oil has coated plants and become trapped; concrete bulkheads, rip rap and piers that may have trapped oil; oiled pilings; gravel or cobble shorelines and rocky shores, where oil has become trapped in pockets; and, public beaches, fisheries, hatcheries, river banks, and other sensitive or high impact sites. The CYTOSOL has been field tested successfully for removing oil from mussel beds and intertidal zones, pilings and concrete rip rap. The CYTOSOL also proved effective in facilitating the removal of oil from the banks and vegetation along an oiled creek.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:

Material Tested	Species	LC50 (ppm)
CYTOSOL	Menidia beryllina Mysidopsis bahia	738.0 96-hr 124.0 48-hr
No. 2 Fuel Oil	Menidia beryllina Mysidopsis bahia	38.9 96-hr 5.9 48-hr
CYTOSOL & No. 2 Fuel Oil (1:10)	Menidia beryllina Mysidopsis bahia	24.3 96-hr 7.0 48-hr
Reference Toxicant (DSS)	Menidia beryllina Mysidopsis bahia	13.8 96-hr 22.2 48-hr

NOTE: This toxicity data was derived with the EPA protocols for dispersants using a blender to emulsify the product into the water for testing organisms. The CYTOSOL emulsion created microdroplets of product which may have had direct physical effects on the test larvae. Since the solubility of the product in water is so low (14 ppm or less), it is probable that the observed effects on the test organisms was caused by larvae having direct contact with droplets of product rather than by a true chemical toxicity from the trace amount of dissolved product. In practice, the CYTOSOL would not be emulsified to any great extent during application. See Section VI of this bulletin for information regarding the manufacturer's recommendations for concentrations and application rates for field use.

b. Effectiveness:
NA

VIII. MICROBIOLOGICAL ANALYSIS
NA

IX. PHYSICAL PROPERTIES
1. Flash Point: 360ºF 2. Pour Point: 10ºF 3. Viscosity: 4.15 CST @ 104ºF 4. Specific Gravity: 0.8877 @ 60ºF 5. pH: Neutral 6. Surface Active Agents: None 7. Solvents: No Petroleum Distillates 8. Additives: CONFIDENTIAL 9. Solubility: 14 ppm in fresh water, 7 ppm in sea water

X. ANALYSIS FOR HEAVY METALS AND CHLORINATED HYDROCARBONS

Compound	Concentration (ppm)
Arsenic	ND
Cadmium	ND
Chromium	ND
Copper	ND
Lead	ND
Mercury	ND
Nickel	ND
Zinc	ND
Cyanide	ND
Chlorinated Hydrocarbons	ND

CYTOSOL

Emergency Response