ECOBIOCLEAN® 100 COSW

1. Application Method: ECOBIOCLEAN® 100 COSW is a multi-faceted bioremediation product that may be applied directly in powder form or diluted with non-chlorinated water to initiate degradation of crude oil (hydrocarbons) at the source of contamination such as on shorelines, vegetation, or land. For powder treatment, apply directly to contamination site via crop dusting implements, farm fertilizer apparatus, and planes for larger areas. For smaller spills where powder form is desired, use fertilizer distributors, hand sprinklers, or other dry nutrient delivery system. For liquid applications and after dilution, apply with any commercially available spray apparatus, such as hand held tanks, back packs, and large mixing tanks with pumping devices. In addition, ECOBIOCLEAN® 100 COSW may be applied via educator systems from vessels, fire trucks, or any other appropriately outfitted vehicle. Spraying/applying to the outer edges of contamination site first and then working inward will help control spreading of spill.
2. Concentration/Application Rate: Concentration rates will vary based upon the type of crude oil spilled, the type of substrate and the amount of contamination spilled, the weathering process, and environmental conditions. ECOBIOCLEAN® 100 COSW contains billions of active hydrocarbon consuming microbes (MC) per gram along with a supporting proprietary complex of nutrients (NA) and enzymes (EA), which together are bio-catalyzing elements that stimulate bioremediation. ECOBIOCLEAN® 100 COSW may be applied to shorelines or land in full strength powder form or diluted using native water supplies as required depending upon the location of environmental contamination (do not use municipal/chlorinated water for mixing). Product may be applied immediately after mixing or within 12 hours via hoses or other spray devices or systems. Dilute 1:10 to 1:30 (1 pound product to 10-30 gallons native water) depending upon the type of contamination. For example, heavy petroleum compounds such as crude oil or bunker fuel will require higher dose of ECOBIOCLEAN® 100 COSW and lighter petroleum compounds will require less. Nutrient additives are not necessary. Within minutes ECOBIOCLEAN® 100 COSW should begin to penetrate the molecular walls initiating bioremediation. ECOBIOCLEAN® 100 COSW provides an inoculum that insures bacterial colonies consume hydrocarbons throughout the remediation process. Within 3-7 days, there should be a noticeable reduction of crude oil contamination and odor while population of microbial strains continue to proliferate. Weathered oils may require longer treatment times. Diluted product can be sprayed from a boat or aircraft to degrade surface oils on soil, beach/sand surface, or vegetation. When dry application is preferred, such as in or around UST/AST and containment pits, ECOBIOCLEAN® 100 COSW can be applied via powder distribution implements as with seeding/fertilizing equipment or dry sprayers and then tilled for more complete distribution of product to contaminant. Be certain to cover the entire contaminated area for thorough treatment starting with perimeter and working to center. Product will quickly begin to break the hydrocarbon structure into organic nutrient substances that aquatic and ground life can feed upon. In some circumstances (such as above) tilling of the ground is advisable to insure higher oxygenation and that product is reaching offending chemical. Within hours, odors should be greatly reduced, as may the hydrocarbon bonding properties helping to prevent adhesion to vegetation, rocks, soil/earth, and wildlife. (Use only non-chlorinated water for dilution.) Estimate area needing treatment. Use 1 pound of product (dilute 1:10 to 1:25 product to gallons of water) per cubic yard of heavy crude oil spilled, or 10 pounds of product per acre of land depending upon the oil type, penetration depth and concentration. Heavier oils may need greater biomass than lighter oils or fuels.
3. Conditions for Use: Product is effective in salt water or fresh water ecosystems including marshlands, rocks, vegetation, sand, and soil (shorelines/land) with no limitations as to usage within temperature parameters above 35ºF and below 100ºF. For greater immediate activity, water temperature for optimal mixing is between 60-80ºF, though you may mix with warmer or colder non-chlorinated water between 45-105ºF if need exists. Water salinity is not a factor as ECOBIOCLEAN® 100 COSW works well with all forms of water. Weathered crude/hydrocarbon contamination or tar-like substances may need to be agitated or tilled/cut for shorter remediation time prior to or with application. For heavy oils use product directly on the spill or up to approximately a 1:10 dilution ratio (1 pound product to 10 gallons of water). Lighter oils would require a 1:10 to 1:30 product to water dilution ratio. In warmer waters and in good agitation during application less product may be required. ECOBIOCLEAN® 100 COSW will work more quickly when adequate oxygen levels exist at contamination site but is viable wherever microbial life can be sustained.

a. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table

1. Listing of each component of the total formulation, other than microorganisms, by chemical name and percentage by weight: CONFIDENTIAL
2. Listing of all microorganisms by species and percentages of each species in the composition of the additive: CONFIDENTIAL
3. Optimum pH, temperature, and salinity ranges for use of the additive:
   pH: range 6.5 to 7.5
   Temperature: range 75°F to 85°F
   Salinity: 0.5 to 15.0%
4. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
   pH: 5.5 and 11.0
   Temperature: 32°F and 120°F
   Salinity: maximum permissible salinity is up to 20% by solution
5. Special nutrient requirements: NA
6. Test results regarding the determination of the presence of the following:
   Salmonella: Negative
   Fecal coliform: Negative
   Shigella: Negative
   Staphylococcus Coagulase positive: Negative
   Beta hemolytic Streptococci: Negative

1. Listing of each component of the total formulation, other than enzymes, by chemical and percentage by weight: CONFIDENTIAL
2. Enzyme(s): CONFIDENTIAL
3. International Union of Biochemistry (I.U.B.) number(s): CONFIDENTIAL
4. Source of enzyme: CONFIDENTIAL
5. Units: No less than 1% and no more than 25% by weight.
6. Specific Activity: CONFIDENTIAL
7. Optimum Conditions:
   pH: 7.0
   Temperature: CONFIDENTIAL
   Salinity ranges for use of the additive: CONFIDENTIAL
   Minimum and maximum pH: 4.5 to 8.5
   Minimum and maximum temperature: CONFIDENTIAL
   Minimum and maximum salinity levels above or below which the effectiveness of the additive is reduced to half its optimum capacity: CONFIDENTIAL
8. Enzyme shelf life: Up to 5 years if unopened and stored properly
9. Enzyme optimum storage conditions: Low moisture environment at temperatures between 40ºF and 115ºF

1. Listing of each component of the total formulation by chemical name and percentage by weight: CONFIDENTIAL
2. Nutrient additive optimum storage conditions: Store product in sealed containers and dry area at temperatures between 40ºF and 120ºF.