JE1058BS

TECHNICAL PRODUCT BULLETIN B-58
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: DECEMBER 3, 2007
“JE1058BS”
LAST COMMUNICATION WITH MANUFACTURER: 3/22/2016
LAST ATTEMPT: 7/26/2021

I. NAME, BRAND, OR TRADEMARK
JE1058BS Type of Product: Bioremediation Agent (Nutrient Additive)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Japan Energy Corporation Business Development Department, Bio Research Center 3-17-35 Niizo-Minami Toda-shi, Saitama 335-8502 Japan Phone: (81) 48-433-2191 Fax: (81) 48-444-3223 Email: saeki@j-energy.co.jp (Hisashi Saeki)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Japan Energy Corporation Business Development Department, Bio Research Center 3-17-35 Niizo-Minami Toda-shi, Saitama 335-8502 Japan Phone: (81) 48-433-2191 Fax: (81) 48-444-3223 Email: saeki@j-energy.co.jp (Hisashi Saeki)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable. 2. Ventilation: Use adequate ventilation. Treat product as a hygroscopic powder. 3. Skin and eye contact; protective clothing; treatment in case of contact: Avoid contact with eyes and do not take internally. Upon contact with eyes, flush with plenty of fresh water. Avoid breathing dust. Normal protective equipment for handling of powder, such as a dust mask and eye shield can be used. 4.a. Maximum storage temperature: 105°F 4.b. Minimum storage temperature: NA 4.c. Optimum storage temperature range: 0°F - 68°F 4.d. Temperatures of phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability: Non-flammable.
2. Ventilation: Use adequate ventilation. Treat product as a hygroscopic powder.
3. Skin and eye contact; protective clothing; treatment in case of contact: Avoid contact with eyes and do not take internally. Upon contact with eyes, flush with plenty of fresh water. Avoid breathing dust. Normal protective equipment for handling of powder, such as a dust mask and eye shield can be used.
4.a. Maximum storage temperature: 105°F
4.b. Minimum storage temperature: NA
4.c. Optimum storage temperature range: 0°F - 68°F
4.d. Temperatures of phase separations and chemical changes: NA

V. SHELF LIFE
The shelf life of the product is approximately 2 years if stored in a cool dry area.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: JE1058BS contains biosurfactant and has an ability to stimulate the biodegradation of oil by indigenous microorganisms. JE1058BS, which is a powder, can be applied using conventional spraying equipment, for example a powder mist duster attached with boom type multi-hole head. 2. Concentration/Application Rate: JE1058BS is applied in a product:oil ratio of 1:10. No pre-mixing or dilution by water is required. 3. Conditions for Use: JE1058BS may be used in a wide range of environmental conditions of temperature and salinities where natural populations of oil-degrading microbes are active. The mechanical removal of as much oil as possible should be done before the product is introduced to the slick.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
JE1058BS contains biosurfactant and has an ability to stimulate the biodegradation of oil by indigenous microorganisms. JE1058BS, which is a powder, can be applied using conventional spraying equipment, for example a powder mist duster attached with boom type multi-hole head.
2. Concentration/Application Rate:
JE1058BS is applied in a product:oil ratio of 1:10. No pre-mixing or dilution by water is required.
3. Conditions for Use:
JE1058BS may be used in a wide range of environmental conditions of temperature and salinities where natural populations of oil-degrading microbes are active. The mechanical removal of as much oil as possible should be done before the product is introduced to the slick.

VII. TOXICITY AND EFFECTIVENESS

a. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:
Summary Data Table

Days	Product 3 Reps/Prod	Total Mean Alkanes (ppm)	28 Days RED%	Total Mean Aromatics (ppm)	28 Days RED%
0	Control	48,213		11,413
	Nutrient	51,833		13,056
	JE1058BS	52,280		13,211
7	Control	48,030		11,108
	Nutrient	33,423		11,755
	JE1058BS	13,453		10,188
28	Control	48,257	0	10,429	8.6
	Nutrient	16,953	67.3	11,837	9.3
	JE1058BS	3,854	92.6	8,061	39.0

Results of Gravimetric Analysis: DATA MISSING

Percentage (%) Decrease in Weight of Oil on Day 28
Control: 5.9%
Nutrient: 60.0%
Product: 81.8%

Material Tested	SPECIES	LC50 (ppm)
JE1058BS	Menidia beryllina Mysidopsis bahia	91.70 96-hr 72.09 48-hr
No. 2 Fuel Oil	Menidia beryllina Mysidopsis bahia	5.56 96-hr 2.19 48-hr
JE1058BS & No. 2 Fuel Oil (1:10)	Menidia beryllina Mysidopsis bahia	8.68 96-hr 2.44 48-hr
Reference Toxicant (SLS)	Menidia beryllina Mysidopsis bahia	12.23 96-hr 11.70 48-hr

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: NA 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: Store in dry location and avoid contact with moisture 4. Special nutrient requirements: NA 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive: NA
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
Store in dry location and avoid contact with moisture
4. Special nutrient requirements: NA
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

JE1058BS

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