S-200C

TECHNICAL PRODUCT BULLETIN #B-56
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: JULY 24, 2002
"S-200C"

I. NAME, BRAND, OR TRADEMARK
S-200C Type of Product: Bioremediation Agent (Nutrient Additive)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
RBL Environmental, LLC* 1311 Dorothy Avenue Phoenixville, PA 19460 Phone: (610) 520-7665 E-mail: jim.lynn@iepusa.com (Mr. James Lynn) (*RBL Environmental, LLC is the manufacturer and marketer for International Environmental Products)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
RBL Environmental, LLC 1311 Dorothy Avenue Phoenixville, PA 19460 Phone: (610) 520-7665 E-mail: jim.lynn@iepusa.com (Mr. James Lynn)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable. 2. Ventilation: Ventilation of the work place is not necessary. 3. Skin and eye contact; protective clothing; treatment in case of contact: In case of contact with skin or eyes, wash with plenty of water. 4.a. Maximum storage temperature: 132ºF 4.b. Minimum storage temperature: 32ºF 4.c. Optimum storage temperature range: 55ºF - 85ºF 4.d. Temperatures of phase separations: <32ºF and >140ºF

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Non-flammable.
2. Ventilation:
Ventilation of the work place is not necessary.
3. Skin and eye contact; protective clothing; treatment in case of contact:
In case of contact with skin or eyes, wash with plenty of water.
4.a. Maximum storage temperature: 132ºF
4.b. Minimum storage temperature: 32ºF
4.c. Optimum storage temperature range: 55ºF - 85ºF
4.d. Temperatures of phase separations: <32ºF and >140ºF

V. SHELF LIFE
Recommended shelf life of S-200C is 10 years if kept in the unopened, original container.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: S-200C is a light amber liquid, and as such can be sprayed with traditional liquid spraying equipment, such as pressurized sprayers or backpack sprayers. 2. Concentration/Application Rate: The recommended application rate is approximately 10% by volume of the S-200C as compared to the hydrocarbon being remediated. In other words, use 1 pound of S-200C for every 10 pounds of hydrocarbon you are remediating. For applications on soil contaminated below 1 foot, the soil is turned and the material is applied. This allows air to circulate and thereby maintains an aerobic condition. On a surface application, the amount to use is approximately 0.1 to 1 pound of S-200C for every square yard of surface area. The amount sprayed is dependent on the amount of hydrocarbon on the surface you are spraying. To remediate a sheen on water, the same application ratio for a surface application is used. 3. Conditions for Use: S-200C is a bioremediation accelerator used for the remediation of hydrocarbon spills or leaks. It is a non-intrusive, cost effective remedy for the cleanup of these hydrocarbons. These hydrocarbons include but are not limited to gasoline, No. 2 to No. 6 diesel fuel, jet fuels, kerosene, lubricating oils, hydraulic oils, and crude oils.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
S-200C is a light amber liquid, and as such can be sprayed with traditional liquid spraying equipment, such as pressurized sprayers or backpack sprayers.
2. Concentration/Application Rate:
The recommended application rate is approximately 10% by volume of the S-200C as compared to the hydrocarbon being remediated. In other words, use 1 pound of S-200C for every 10 pounds of hydrocarbon you are remediating.
For applications on soil contaminated below 1 foot, the soil is turned and the material is applied. This allows air to circulate and thereby maintains an aerobic condition.
On a surface application, the amount to use is approximately 0.1 to 1 pound of S-200C for every square yard of surface area. The amount sprayed is dependent on the amount of hydrocarbon on the surface you are spraying.
To remediate a sheen on water, the same application ratio for a surface application is used.
3. Conditions for Use:
S-200C is a bioremediation accelerator used for the remediation of hydrocarbon spills or leaks. It is a non-intrusive, cost effective remedy for the cleanup of these hydrocarbons. These hydrocarbons include but are not limited to gasoline, No. 2 to No. 6 diesel fuel, jet fuels, kerosene, lubricating oils, hydraulic oils, and crude oils.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity

Material Tested	Species	LC50 (ppm)
S-200C	Menidia beryllina Mysidopsis bahia	39.69 96-hr 21.33 48-hr
No. 2 Fuel Oil	Menidia beryllina Mysidopsis bahia	35.36 96-hr 35.36 48-hr
S-200C & No. 2 Fuel Oil (1:10)	Menidia beryllina Mysidopsis bahia	25.33 96-hr 17.67 48-hr
Reference Toxicant (SDS)	Menidia beryllina Mysidopsis bahia	NA 96-hr 22.96 48-hr

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL S-200C	27666 29333	0 0	5600 5666	0 0
7	CONTROL S-200C	21333 11000	0 0	4333 4400	0 0
28	CONTROL S-200C	18771 660	32 98	5597 5073	0.05 10.4

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28

Control	Product (001)
-18.57%	27.82%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: S-200C is a nutrient bioremediation accelerator and does not contain bacterial cultures or amendments. However, with the addition of S-200C, the microbiological data shows a continued viability of microbial incubations over time. 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 7.0 Temperature: 86ºF Salinity: Fresh to salt water 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 4.5 - 10.0 Temperature: 50ºF - 120ºF Salinity: 0% - 10% 4. Special nutrient requirements: NA 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
S-200C is a nutrient bioremediation accelerator and does not contain bacterial cultures or amendments. However, with the addition of S-200C, the microbiological data shows a continued viability of microbial incubations over time.
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 7.0
Temperature: 86ºF
Salinity: Fresh to salt water
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 4.5 - 10.0
Temperature: 50ºF - 120ºF
Salinity: 0% - 10%
4. Special nutrient requirements:
NA
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
1. Flash Point: > 212ºF 2. Pour Point: 40ºF 3. Viscosity: 100cp @ 70ºF 4. Specific Gravity: 0.996 5. pH: 4.5 6. Surface Active Agents: NA 7. Solvents: NA 8. Additives: NA 9. Solubility in Water: Dispersable

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
S-200C does not contain any heavy metals, cyanide, or chlorinated hydrocarbons.

S-200C

Emergency Response