SUMP SAFE BIO-RECLAIM

TECHNICAL PRODUCT BULLETIN: B-69
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: OCTOBER 13, 2011
“SUMP SAFE BIO-RECLAIM”
LAST COMMUNICATION WITH MANUFACTURER: 4/27/2016
LAST ATTEMPT: 8/02/2021

I. NAME, BRAND, OR TRADEMARK
SUMP SAFE BIO-RECLAIM Type of Product: Bioremediation Agent (Biological Additive/Microbiological Culture)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Teamwork Distributing P.O. BOX 2506 Stony Plain, Alberta T7Z 1X1 Phone: (780) 968-5367 (Plant) Mobile: (780) 238-2741 Fax: (780) 958-9070 E-mail: updated information required (Mr. Marlin Rudolph)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
PNE Corporation 55 International Way Longview, WA 98632 Phone: (360) 423-2245 Fax: (360) 423-2272 E-mail: garyh@pnecorp.com Website: http://www.pnecorp.com/ (Mr. Gary Healea)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable (DOT: Non-hazardous) 2. Ventilation: No special requirements 3. Skin and eye contact; protective clothing; treatment in case of contact: No special equipment or clothing required, however goggles are recommended. If eye or skin irritation occurs, flush with plenty of fresh water. 4.a. Maximum storage temperature: 110°F maximum, must be used within 24 hours from constitution 4.b. Minimum storage temperature: 45°F 4.c. Optimum storage temperature range: 60°F to 90°F 4.d. Temperatures of phase separation and chemical changes: Stable

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability: Non-flammable (DOT: Non-hazardous)
2. Ventilation: No special requirements
3. Skin and eye contact; protective clothing; treatment in case of contact: No special equipment or clothing required, however goggles are recommended. If eye or skin irritation occurs, flush with plenty of fresh water.
4.a. Maximum storage temperature: 110°F maximum, must be used within 24 hours from constitution
4.b. Minimum storage temperature: 45°F
4.c. Optimum storage temperature range: 60°F to 90°F
4.d. Temperatures of phase separation and chemical changes: Stable

V. SHELF LIFE
The material must be used within 24-48 hours of constitution of the powdered bacteria portion in water. The dry material must be kept at -20°C and is stable for two years at this temperature. Once sent to the field, the material may be stored on ice for up to two weeks prior to constitution.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: Product may be applied by the usual methods. For smaller spills a drum pump with sprayer may be used, mixing with fresh water typically. The concentrate is used at 25:1 dilution rate with water and is typically applied at a rate of 6 gallons per cubic meter of soil. It is important that the soil be broken up into small clumps (rototilling is acceptable) to ensure effective application. The soil is left and biodegradation is expected to complete in approximately 12 weeks. Analysis using approved sampling procedures is performed to confirm biodegradation. For larger volume projects, any auger based soil homogenizer is employed to break up large clumps of soil, which are dug out from the contaminated area using a standard hoe. The broken up soil is taken up a conveyor and has the diluted concentrate (25:1) at a rate of 6 gallons per cubic meter of soil. Piles up to 20 feet high may be formed. The soil is left and biodegradation is expected to be complete in approximately 12-18 weeks. Analysis using approved sampling procedures is performed to confirm biodegradation. Proper safety rules must be employed to ensure that any holes dug out are not accessible to employees or unauthorized personnel. For oil spills on open water, it is recommended that a 25:1 dilution of the product be applied via spraying. In this way, the bacteria can contact the floating oil and immediately begin to form a bio-film. The spray would also be applied on any soil contaminated on banks, etc. It is important to ensure complete coverage of the water/oily surface and soil during spray application. Degradation will be visible visually and can be confirmed by Fats, Oil and Grease (FOG) analysis. For smaller, contained volumes of oily water (typically emulsified, white water), it is recommended that sufficient concentrate be added to the contaminated water such that it results in a 2.5% concentration of the concentrate in the contaminated water. Preferably, aeration can be performed, using even a small, fish tank aeration unit. Visual evidence of biodegradation should be apparent within one week or less and can be confirmed by FOG analysis. 2. Concentration/Application Rate: The concentrate is used at 25:1 dilution rate with water and is typically applied at a rate of 6 gallons per cubic meter of soil. 3. Conditions for Use: The product can be used in fresh or salt water and may be applied at temperatures between 40°F and 120°F. However, the product is most effective when applied at water temperatures between 70°F-90°F. Further, the product is effective on fresh spills or aged hydrocarbons. Note for preparation of the product to be applied while in the field: all components listed in the product are packaged as a complete unit and are applied as such. The microbial portion of the product is supplied in bags which are either drum (55 gallon) or pail (5 gallon) sizes. Fresh water, if available, is added to fill the bag (bags are placed in pails or open headed drums) to 55 gallon of 5 gallons respectively. Depending upon which of these are being constituted with water, a high-density polyethylene (HDPE) bottle with the appropriate quantity of surfactant is added into the container (bottles are labeled Bio-Reclaim Surfactant – Drum Size (or Pail Size)). In the same way, HDPE containers with appropriate amounts of sodium nitrite are added and are labeled Bio-Reclaim Salt – Drum Size (or Pail Size)).

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method: Product may be applied by the usual methods. For smaller spills a drum pump with sprayer may be used, mixing with fresh water typically. The concentrate is used at 25:1 dilution rate with water and is typically applied at a rate of 6 gallons per cubic meter of soil. It is important that the soil be broken up into small clumps (rototilling is acceptable) to ensure effective application. The soil is left and biodegradation is expected to complete in approximately 12 weeks. Analysis using approved sampling procedures is performed to confirm biodegradation.

For larger volume projects, any auger based soil homogenizer is employed to break up large clumps of soil, which are dug out from the contaminated area using a standard hoe. The broken up soil is taken up a conveyor and has the diluted concentrate (25:1) at a rate of 6 gallons per cubic meter of soil. Piles up to 20 feet high may be formed. The soil is left and biodegradation is expected to be complete in approximately 12-18 weeks. Analysis using approved sampling procedures is performed to confirm biodegradation. Proper safety rules must be employed to ensure that any holes dug out are not accessible to employees or unauthorized personnel.

For oil spills on open water, it is recommended that a 25:1 dilution of the product be applied via spraying. In this way, the bacteria can contact the floating oil and immediately begin to form a bio-film. The spray would also be applied on any soil contaminated on banks, etc. It is important to ensure complete coverage of the water/oily surface and soil during spray application. Degradation will be visible visually and can be confirmed by Fats, Oil and Grease (FOG) analysis.

For smaller, contained volumes of oily water (typically emulsified, white water), it is recommended that sufficient concentrate be added to the contaminated water such that it results in a 2.5% concentration of the concentrate in the contaminated water. Preferably, aeration can be performed, using even a small, fish tank aeration unit. Visual evidence of biodegradation should be apparent within one week or less and can be confirmed by FOG analysis.

2. Concentration/Application Rate: The concentrate is used at 25:1 dilution rate with water and is typically applied at a rate of 6 gallons per cubic meter of soil.

3. Conditions for Use: The product can be used in fresh or salt water and may be applied at temperatures between 40°F and 120°F. However, the product is most effective when applied at water temperatures between 70°F-90°F. Further, the product is effective on fresh spills or aged hydrocarbons. Note for preparation of the product to be applied while in the field: all components listed in the product are packaged as a complete unit and are applied as such. The microbial portion of the product is supplied in bags which are either drum (55 gallon) or pail (5 gallon) sizes. Fresh water, if available, is added to fill the bag (bags are placed in pails or open headed drums) to 55 gallon of 5 gallons respectively. Depending upon which of these are being constituted with water, a high-density polyethylene (HDPE) bottle with the appropriate quantity of surfactant is added into the container (bottles are labeled Bio-Reclaim Surfactant – Drum Size (or Pail Size)). In the same way, HDPE containers with appropriate amounts of sodium nitrite are added and are labeled Bio-Reclaim Salt – Drum Size (or Pail Size)).

VII. TOXICITY AND EFFECTIVENESS

a. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT PRODUCT	40,770 41,329 40,609	0 0 0	5,229 5,326 5,384	0 0 0
7	CONTROL NUTRIENT PRODUCT	37,530 21,788 38,354	0 0 0	4,824 4,634 5,298	0 0 0
28	CONTROL NUTRIENT PRODUCT	31,456 502 21,356	22.8 98.8 47.4	3,692 3,052 3,742	24.2 42.7 49.0

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 6.36%
Nutrient: 33.00%
Product: 3.00%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of each component of the total formulation, other than microorganisms, by chemical name and percentage by weight: CONFIDENTIAL 2. Listing of all microorganisms by species and percentages of each species in the composition of the additive: CONFIDENTIAL 3. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 6-8 Temperature: 90°F Salinity: Not applicable 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 4.8 – 9 Temperature: 59°F – 104°F Salinity: Not applicable 4. Special nutrient requirements: A combination of 70 percent live active yeast mixed with 30 percent fine ground corn cob is used as a nutrient. In a 55 gallon drum of concentrate, approximately 1.1 kg of this mix will be found (relative to the 1.1 kg, the actual bacteria represent the negligible mass of several grams). There are no storage requirements for the nutrient portion of the formula; however, as they are in a homogenous mixture with the bacteria, they must be stored as described in Part V of this bulletin. 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of each component of the total formulation, other than microorganisms, by chemical name and percentage by weight: CONFIDENTIAL
2. Listing of all microorganisms by species and percentages of each species in the composition of the additive: CONFIDENTIAL
3. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 6-8
Temperature: 90°F
Salinity: Not applicable
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 4.8 – 9
Temperature: 59°F – 104°F
Salinity: Not applicable
4. Special nutrient requirements: A combination of 70 percent live active yeast mixed with 30 percent fine ground corn cob is used as a nutrient. In a 55 gallon drum of concentrate, approximately 1.1 kg of this mix will be found (relative to the 1.1 kg, the actual bacteria represent the negligible mass of several grams). There are no storage requirements for the nutrient portion of the formula; however, as they are in a homogenous mixture with the bacteria, they must be stored as described in Part V of this bulletin.
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS OF HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

SUMP SAFE BIO-RECLAIM

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