SYSTEM E.T. 20

TECHNICAL PRODUCT BULLETIN #B-45
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: JANUARY 28, 1993
REVISED LISTING DATE: NOVEMBER 14, 1995
"SYSTEM E.T. 20"
LAST COMMUNICATION WITH MANUFACTURER: 8/09/2016
LAST ATTEMPT: 8/02/2021

I. NAME, BRAND, OR TRADEMARK
SYSTEM E.T. 20 Type of Product: Biological Additive (formerly MCW.B 20)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Environmental Restoration Services 9211 Lakewood Drive Windsor, CA 95492 E-mail: ERS.BTI@gmail.com Phone: (619) 253-0664 (Mr. John Chase)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Environmental Restoration Services 14384 Highland Valley Road Escondido, CA 92025 E-mail: ERS.BTI@gmail.com Phone: (619) 253-0664 (Mr. John Chase)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: SYSTEM E.T. 20 is non-flammable 2. Ventilation: No special ventilation is required under normal use. 3. Skin and eye contact; protective clothing; treatment in case of contact: In its primary form, no special handling or storage is required. Avoid excessive inhalation, and protect the nose and mouth with a dust protection mask. Wear protective gloves, and wash hands with soap and water after handling the product. 4.a. Maximum storage temperature: 4.b. Minimum storage temperature: 4.c. Optimum storage temperature range: 4ºC - 20ºC (39ºF - 68ºF). Place in a dry area, shaded from sunlight. 4.d. Temperatures of phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
SYSTEM E.T. 20 is non-flammable
2. Ventilation:
No special ventilation is required under normal use.
3. Skin and eye contact; protective clothing; treatment in case of contact:
In its primary form, no special handling or storage is required. Avoid excessive inhalation, and protect the nose and mouth with a dust protection mask. Wear protective gloves, and wash hands with soap and water after handling the product.
4.a. Maximum storage temperature:
4.b. Minimum storage temperature:
4.c. Optimum storage temperature range: 4ºC - 20ºC (39ºF - 68ºF). Place in a dry area, shaded
from sunlight.
4.d. Temperatures of phase separations and chemical changes: NA

V. SHELF LIFE
2 years if maintained at 4ºC (39ºF).

VI. RECOMMENDED APPLICATION PROCEDURE
SYSTEM E.T. 20 can be used on a broad range of hydrocarbon compounds (including PAH's) found in open and closed water systems (salt or fresh) or in soil (beach, sands, or inland soil types). SYSTEM E.T. 20 protocols can be used with indigenous and introduced bacteria. SYSTEM E.T. 20 is an oleophilic, non-water soluble nutrient, which releases nitrogen and phosphorus enzymatically, thus allowing SYSTEM E.T. 20 to be used in water and soil applications including: Conditions requiring fast degradation rates due to regulations, limited space or cost; Conditions where weathered or heavy oils, sludges or PAH's exist that require consistently high populations of hydrocarbon degrading bacteria to metabolize these long or more complex carbon chains; Tropical or sub-tropical conditions that support large, indigenous, non-beneficial bacterial populations that would otherwise dilute the effectiveness of standard bioaugmentation approaches; Aqueous conditions in which soluble or time release nutrient compounds would be washed away or diluted, i.e., beaches, frequent rains, floods, streams, stream banks, estuaries and areas affected by tides; Sensitive environmental conditions that demand low or no toxicity; Toxicity in soil or water during bioremediation may be stimulated by adding broadly available nutrients which may activate disease carrying bacilli or pathogens. Controlled nutrient release to SYSTEM E.T. 20 bacteria prevents over-nitrification; Conditions requiring the elimination of oil discoloration caused by the presence of polar fractions; and Conditions where salt water conditions exist or where only salt water is available for bacteria application. 1. Application Methods: Biopile Land farm Pump and Treat Bio reactor Bio slurry In situ Windrow Topical application to discolored rocks or soil Topical application to beaches 2. Concentration/Application Rate: Concentration and rate of application will vary from site to site depending upon the type of contaminant and the area contaminated. Contact ERS for specific information. 3. Conditions for Use: Before applying SYSTEM E.T. 20, ERS recommends that the site be evaluated for its physical and chemical soil characteristics (i.e., pH, salinity, conductivity, physical parameters, moisture content, hydrocarbon type and concentration, nitrogen content, etc.), and toxicity of both the soil and the contaminant, by biological screening tests. Basic biological requirements of SYSTEM E.T. 20, site conditions and SYSTEM E.T. 20 capabilities dictate the appropriate procedures for application of SYSTEM E.T. 20. Biological requirements for SYSTEM E.T. 20 bacteria are nutrients, adequate oxygen supply, hydrocarbon food source, neutral pH and a 15% minimum moisture level for soil remediation applications.

VI. RECOMMENDED APPLICATION PROCEDURE

SYSTEM E.T. 20 can be used on a broad range of hydrocarbon compounds (including PAH's) found in open and closed water systems (salt or fresh) or in soil (beach, sands, or inland soil types). SYSTEM E.T. 20 protocols can be used with indigenous and introduced bacteria. SYSTEM E.T. 20 is an oleophilic, non-water soluble nutrient, which releases nitrogen and phosphorus enzymatically, thus allowing SYSTEM E.T. 20 to be used in water and soil applications including:

Conditions requiring fast degradation rates due to regulations, limited space or cost;
Conditions where weathered or heavy oils, sludges or PAH's exist that require consistently high populations of hydrocarbon degrading bacteria to metabolize these long or more complex carbon chains;
Tropical or sub-tropical conditions that support large, indigenous, non-beneficial bacterial populations that would otherwise dilute the effectiveness of standard bioaugmentation approaches;
Aqueous conditions in which soluble or time release nutrient compounds would be washed away or diluted, i.e., beaches, frequent rains, floods, streams, stream banks, estuaries and areas affected by tides;
Sensitive environmental conditions that demand low or no toxicity; Toxicity in soil or water during bioremediation may be stimulated by adding broadly available nutrients which may activate disease carrying bacilli or pathogens. Controlled nutrient release to SYSTEM E.T. 20 bacteria prevents over-nitrification;
Conditions requiring the elimination of oil discoloration caused by the presence of polar fractions; and
Conditions where salt water conditions exist or where only salt water is available for bacteria application.

1. Application Methods:
Biopile
Land farm
Pump and Treat
Bio reactor
Bio slurry
In situ
Windrow
Topical application to discolored rocks or soil
Topical application to beaches
2. Concentration/Application Rate:
Concentration and rate of application will vary from site to site depending upon the type of contaminant and the area contaminated. Contact ERS for specific information.
3. Conditions for Use:
Before applying SYSTEM E.T. 20, ERS recommends that the site be evaluated for its physical and chemical soil characteristics (i.e., pH, salinity, conductivity, physical parameters, moisture content, hydrocarbon type and concentration, nitrogen content, etc.), and toxicity of both the soil and the contaminant, by biological screening tests.
Basic biological requirements of SYSTEM E.T. 20, site conditions and SYSTEM E.T. 20 capabilities dictate the appropriate procedures for application of SYSTEM E.T. 20.
Biological requirements for SYSTEM E.T. 20 bacteria are nutrients, adequate oxygen supply, hydrocarbon food source, neutral pH and a 15% minimum moisture level for soil remediation applications.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT SYSTEM E.T. 20	29959.5 29008.5 30476.1	0 0 0	5391.8 5163.0 5311.3	0 0 0
7	CONTROL NUTRIENT SYSTEM E.T. 20	33471.3 22723.7 3837.1	0 0 0	5720.9 5050.1 2928.8	0 0 0
28	CONTROL NUTRIENT SYSTEM E.T. 20	30997.4 1103.9 261.9	0 96.2 99.1	5383.9 4582.4 1188.0	0.2 11.2 77.0

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 0%
Nutrient: 19%
Product (Stm ET 20): 18%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentage in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 7.5 (6.5 to 8.5) Temperature: 4ºC to 35ºC (39ºF to 95ºF) Salinity: Fresh to 2.5% (average ocean salt level) 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: < 6.5 and > 8.5 Temperature: < 5ºC and > 35ºC (< 41ºF and > 95ºF) Salinity: > 3% 4. Special nutrient requirements: Recommendation is for a non-soluble nutrient. The specific types of nutrients will be dependent on the site requirements. 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentage in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 7.5 (6.5 to 8.5)
Temperature: 4ºC to 35ºC (39ºF to 95ºF)
Salinity: Fresh to 2.5% (average ocean salt level)
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: < 6.5 and > 8.5
Temperature: < 5ºC and > 35ºC (< 41ºF and > 95ºF)
Salinity: > 3%
4. Special nutrient requirements:
Recommendation is for a non-soluble nutrient. The specific types of nutrients will be dependent on the site requirements.
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS OF HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

SYSTEM E.T. 20

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