VB591™, VB997™, BINUTRIX®

TECHNICAL PRODUCT BULLETIN #B-42
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: JANUARY 3, 1992
REVISED LISTING DATE: FEBRUARY 5, 1997
UPDATED LISTING LETTER: OCTOBER 7, 2020
VB591™, VB997™, BINUTRIX®

I. NAME, BRAND, OR TRADEMARK
VB591™, VB997™, BINUTRIX® - Patented, partial encapsulated oleophilic (oil-loving) nutrient Type of Product: Biological Additive (a nutrient additive that contains no microorganisms)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
BioNutraTech Inc. 13647 Pemberwick Park Lane Houston, TX 77070 Phone: (800) 567-5432 Phone: (281) 354-5900 Email: shruza@bionutratech.com Web Site: http://www.bionutratech.com Mobile: (713) 301-0254 (Ms. Sandra Hruza)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
BioNutraTech Inc. 13647 Pemberwick Park Lane Houston, TX 77070 Phone: (800) 567-5432 Phone: (281) 354-5900 Email: shruza@bionutratech.com Web Site: http://www.bionutratech.com (Ms. Sandra Hruza)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable 2. Ventilation: Normal ventilation is adequate 3. Skin and eye contact; protective clothing; treatment in case of contact: Normal precautions and protective equipment for handling any type of powder, such as dust protectors and eye shield. Avoid contact with eyes and do not take internally. Upon contact with eyes, flush with water immediately for a minimum of 15 minutes. If redness or irritation continues, contact a physician. Avoid breathing dust. 4.a. Maximum storage temperature: >140ºF 4.b. Minimum storage temperature: NA 4.c. Optimum storage temperature range: 50ºF to 80ºF 4.d. Temperature phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Non-flammable
2. Ventilation:
Normal ventilation is adequate
3. Skin and eye contact; protective clothing; treatment in case of contact:
Normal precautions and protective equipment for handling any type of powder, such as dust protectors and eye shield.
Avoid contact with eyes and do not take internally. Upon contact with eyes, flush with water immediately for a minimum of 15 minutes. If redness or irritation continues, contact a physician. Avoid breathing dust.
4.a. Maximum storage temperature: >140ºF
4.b. Minimum storage temperature: NA
4.c. Optimum storage temperature range: 50ºF to 80ºF
4.d. Temperature phase separations and chemical changes: NA

V. SHELF LIFE
The shelf life is approximately three years if kept dry. Store in dry location and avoid contact with moisture. Prolonged storage may result in the formation of soft clumps which are easily broken by mechanical disruption.

VI. RECOMMENDED APPLICATION PROCEDURE
VB591™, VB997™, and BINUTRIX® is a powder, and can be applied using conventional powder spraying equipment. No pre-mixing or dilution is required. Soft clumps may form after prolonged storage, these clumps are easily broken by mechanical mixing. 1. Application Method: Application for localized spills can be done using hand-held pressurized dust blowers. For inland waterways, ship channels, marinas or coastal wetlands, large dust blowers mounted on barges or ships can be employed to apply the product. Application by aircraft using conventional dust spraying systems is recommended for treatment of large uncontained spreading spills or spills in open waters or at sea. 2. Concentration/Application Rate: Recommended initial application rate is 5 to 15 pounds of VB591™, VB997™, and BINUTRIX® per barrel of spilled oil. Follow-up applications at 48-72 hour intervals should be adjusted to allow for reduction in oil due to clean-up activities and natural loss by evaporation, droplet formation and dispersion and microbial activity. Applications should not exceed 250 pounds per acre per application. 3. Conditions for Use: VB591™, VB997™, and BINUTRIX® should be applied to spilled oil as soon as possible following spillage to stimulate natural oil utilizing microbial populations to maximize biodegradation activity. Application of VB591™, VB997™, and BINUTRIX® to spilled oil does not significantly alter the physical consistency of the spilled oil, and as such will not adversely affect conventional cleanup activities, nor will conventional and removal activities adversely affect the activity of VB591™, VB997™, and BINUTRIX®. Dispose of waste in accordance with local, state, and federal regulations. Information regarding use of VB591™, VB997™, and BINUTRIX® in conjunction with chemical dispersing agents is at present not available.

VI. RECOMMENDED APPLICATION PROCEDURE

VB591™, VB997™, and BINUTRIX® is a powder, and can be applied using conventional powder spraying equipment. No pre-mixing or dilution is required. Soft clumps may form after prolonged storage, these clumps are easily broken by mechanical mixing.
1. Application Method:
Application for localized spills can be done using hand-held pressurized dust blowers. For inland waterways, ship channels, marinas or coastal wetlands, large dust blowers mounted on barges or ships can be employed to apply the product. Application by aircraft using conventional dust spraying systems is recommended for treatment of large uncontained spreading spills or spills in open waters or at sea.
2. Concentration/Application Rate:
Recommended initial application rate is 5 to 15 pounds of VB591™, VB997™, and BINUTRIX® per barrel of spilled oil. Follow-up applications at 48-72 hour intervals should be adjusted to allow for reduction in oil due to clean-up activities and natural loss by evaporation, droplet formation and dispersion and microbial activity. Applications should not exceed 250 pounds per acre per application.
3. Conditions for Use:
VB591™, VB997™, and BINUTRIX® should be applied to spilled oil as soon as possible following spillage to stimulate natural oil utilizing microbial populations to maximize biodegradation activity.
Application of VB591™, VB997™, and BINUTRIX® to spilled oil does not significantly alter the physical consistency of the spilled oil, and as such will not adversely affect conventional cleanup activities, nor will conventional and removal activities adversely affect the activity of VB591™, VB997™, and BINUTRIX®. Dispose of waste in accordance with local, state, and federal regulations.
Information regarding use of VB591™, VB997™, and BINUTRIX® in conjunction with chemical dispersing agents is at present not available.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT VB591™, VB997™, and BINUTRIX®	31041.7 28251.8 28813.8	0 0 0	973.1 976.6 932.8	0 0 0
7	CONTROL NUTRIENT VB591™, VB997™, and BINUTRIX®	31436.33 20728.3 14637.4	0 26.6 49.2	990.5 619.1 733.1	0 36.6 21.4
28	CONTROL NUTRIENT VB591™, VB997™, and BINUTRIX®	32465.8 1787.2 937.97	0 93.7 96.8	925.7 722.6 290.8	0 26.0 73.1

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: < 1%
Nutrient: 17.6%
Product (VB591™, VB997™, and BINUTRIX®): 18.0%

VIII. MICROBIOLOGICAL ANALYSIS
VB591™, VB997™, and BINUTRIX® is an oleophilic nutrient additive and contains no preserved natural or mutated microorganisms of any type. 1. Listing of all microorganisms by species and percentage in the composition: None. 2. Optimum pH, temperature, and salinity ranges for use of the additive: VB591™, VB997™, and BINUTRIX® may be used under any conditions where natural populations of oil-degrading microbes are active. 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: NA Temperature: NA Salinity: NA 4. Special nutrient requirements. None. 5. Test results regarding the determination of the presence of the following: Salmonella: Not applicable fecal coliform: Not applicable Shigella: Not applicable Staphylococcus coagulase positive: Not applicable Beta hemolytic Streptococci: Not applicable

VIII. MICROBIOLOGICAL ANALYSIS

VB591™, VB997™, and BINUTRIX® is an oleophilic nutrient additive and contains no preserved natural or mutated microorganisms of any type.
1. Listing of all microorganisms by species and percentage in the composition:
None.
2. Optimum pH, temperature, and salinity ranges for use of the additive:
VB591™, VB997™, and BINUTRIX® may be used under any conditions where natural populations of oil-degrading microbes are active.
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: NA
Temperature: NA
Salinity: NA
4. Special nutrient requirements.
None.
5. Test results regarding the determination of the presence of the following:
Salmonella: Not applicable
fecal coliform: Not applicable
Shigella: Not applicable
Staphylococcus coagulase positive: Not applicable
Beta hemolytic Streptococci: Not applicable

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS OF HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

VB591™, VB997™, BINUTRIX®

Emergency Response