WMI-2000

TECHNICAL PRODUCT BULLETIN #B-19
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: JUNE 18, 1990
REVISED LISTING DATE: JANUARY 11, 1996
"WMI-2000"
LAST COMMUNICATION WITH MANUFACTURER: 9/07/2016
LAST ATTEMPT: 8/02/2021

I. NAME, BRAND, OR TRADEMARK
WMI-2000 Type of Product: Biological Additive

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
WMI International, Inc. 2104 Brentwood Drive Houston, TX 77019 Phone: updated information required Mobile: updated information required Fax: updated information required Email: wmiintlinc@gmail.com or teri@oxygenorchard.com (Joseph F. Jennings, President)	Mailing Address WMI International, Inc. P.O. Box 130583 Houston, TX 77219 (Mr. Joseph F. Jennings, President)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
WMI International, Inc. 2104 Brentwood Drive Houston, TX 77019 Phone: updated information required Mobile: updated information required Fax: updated information required Email: wmiintlinc@gmail.com or teri@oxygenorchard.com (Joseph F. Jennings, President)	Mailing Address WMI International, Inc. P.O. Box 130583 Houston, TX 77219 (Mr. Joseph F. Jennings, President)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: WMI-2000 is non-flammable. 2. Ventilation: Avoid breathing dust or aerosol due to possibility of sensitization. Use adequate ventilation. 3. Skin and eye contact; protective clothing; treatment in case of contact: Minimize contact with eyes, skin, and clothing. Wash hands thoroughly after handling culture. 4.a. Maximum storage temperature: 100ºF 4.b. Minimum storage temperature: 35ºF 4.c. Optimum storage temperature range: 45ºF - 90ºF Store in a cool, dry location. Keep partially-used containers tightly closed. Prolonged exposure to high temperature and humidity may lower activity of product. 4.d. Temperatures of phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
WMI-2000 is non-flammable.
2. Ventilation:
Avoid breathing dust or aerosol due to possibility of sensitization. Use adequate ventilation.
3. Skin and eye contact; protective clothing; treatment in case of contact:
Minimize contact with eyes, skin, and clothing. Wash hands thoroughly after handling culture.
4.a. Maximum storage temperature: 100ºF
4.b. Minimum storage temperature: 35ºF
4.c. Optimum storage temperature range: 45ºF - 90ºF
Store in a cool, dry location. Keep partially-used containers tightly closed. Prolonged exposure to high temperature and humidity may lower activity of product.
4.d. Temperatures of phase separations and chemical changes: NA

V. SHELF LIFE
The shelf life of WMI-2000 is 2 years if stored at ambient temperatures

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: The culture should be activated for 2 hours in water, when applicable. Conditions for use vary depending on substrate concentration and type, pH, temperature, availability of nutrients, oxygen content, and liquid medium (static or flowing). 2. Concentration/Application Rate: Inoculation concentration is 5-9 billion spores per gram. 3. Conditions for Use: Temperature requirements are between 35ºF and 100ºF. The optimum dissolved oxygen content is 2 ppm at the sludge-to-water interface. Nitrogen and phosphorous are key nutrients which promote cultural growth, and should be maintained at concentrations of 15-20 ppm and 2.5-5 ppm, respectively. The optimum pH range is between 7.0 and 8.0.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
The culture should be activated for 2 hours in water, when applicable. Conditions for use vary depending on substrate concentration and type, pH, temperature, availability of nutrients, oxygen content, and liquid medium (static or flowing).
2. Concentration/Application Rate:
Inoculation concentration is 5-9 billion spores per gram.
3. Conditions for Use:
Temperature requirements are between 35ºF and 100ºF. The optimum dissolved oxygen content is 2 ppm at the sludge-to-water interface. Nitrogen and phosphorous are key nutrients which promote cultural growth, and should be maintained at concentrations of 15-20 ppm and 2.5-5 ppm, respectively. The optimum pH range is between 7.0 and 8.0.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT WMI-2000	2626.7 2763.3 2453.3	0 0 0	1850.0 2030.0 1880.0	0 0 0
7	CONTROL NUTRIENT WMI-2000	2246.7 1920.0 1243.3	0 0 0	1823.3 1666.7 1456.7	0 0 0
28	CONTROL NUTRIENT WMI-2000	2240.0 1210.0 973.3	14.7 56.2 60.3	1866.7 1480.0 1253.0	-0.9 27.0 33.3

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 0%
Nutrient: 25%
Product (WMI-2000): 44%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentage in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additives: Optimum pH: 7.0 Optimum temperature: 45ºF - 90ºF Optimum salinity: Fresh water to sea water 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 6.0 - 8.0 Temperature: 40ºF - 120ºF Salinity range: Fresh to sea water 4. Special nutrient requirements: none 5. Test results regarding the determination of the following: Salmonella - Negative Fecal Coliform - Negative Shigella - Negative Staphylococcus Coagulase positive - Negative Beta hemolytic streptococcus - Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentage in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additives:
Optimum pH: 7.0
Optimum temperature: 45ºF - 90ºF
Optimum salinity: Fresh water to sea water
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 6.0 - 8.0
Temperature: 40ºF - 120ºF
Salinity range: Fresh to sea water
4. Special nutrient requirements:
none
5. Test results regarding the determination of the following:
Salmonella - Negative
Fecal Coliform - Negative
Shigella - Negative
Staphylococcus Coagulase positive - Negative
Beta hemolytic streptococcus - Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

WMI-2000

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