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Final National Level Listed Species Biological Evaluation for Glyphosate

In November 2021, EPA released the final Biological Evaluation (BE) assessing risks to listed species from labeled uses of glyphosate. The term "listed species" refers to those that are federally listed as endangered or threatened, as well as experimental populations and species that are proposed and candidates for listing. The final BE was conducted according to the Revised Method for National Level Listed Species Biological Evaluations of Conventional Pesticides.   

The Revised Method described in this document incorporates:

  • Recommendations from the National Research Council of the National Academy of Sciences (NAS);
  • EPA’s “lessons learned” during the first three pilot BEs;
  • Public comments provided through
    • stakeholder meetings,
    • the docket on the draft BEs for chlorpyrifos, diazinon and malathion, and 
    • the docket on the proposed Revised Method;
  • Comments received during consultation with federally recognized tribes; and
  • Input provided by U.S. Fish and Wildlife Service, National Marine Fisheries Service and the U.S. Department of Agriculture.

The final BE for glyphosate is provided below. The models and tools used to conduct this BE can be found here.

EPA solicited public comments on the draft BE for glyphosate, which was released in November 2020, and prepared a response to comments document. The response to comments (RTC) on the draft BEs for atrazine, simazine, and glyphosate is provided here:  Response to Comments Document (pdf) (572.32 KB)  

  • RTC Attachment 1: Review of toxicity endpoints used in draft BEs (atrazine, simazine and glyphosate) 
  • RTC Attachment 2: Results of additional MAGtool runs for atrazine BE 
  • RTC Attachment 3: MAGtool files for atrazine
  • RTC Attachment 4: Updated glyphosate estimated environmental concentrations (EECs)

Read the Frequently Asked Questions for the glyphosate, atrazine, and simazine final BEs (pdf) (144.48 KB)

On this page:

  • Final Glyphosate Executive Summary
  • Chapter 1: Final Glyphosate Problem Formulation
    • Attachments
    • Appendices
  • Chapter 2: Final Glyphosate Effects Characterization
    • Attachments
    • Appendices
  • Chapter 3: Final Glyphosate Exposure Characterization
    • Attachments
    • Appendices
  • Chapter 4: Final Glyphosate Effects Determinations
    • Attachments
    •  Appendices

Final Glyphosate Executive Summary (DOCX)

Chapter 1: Final Glyphosate Problem Formulation (DOCX)

Attachments

  • ATTACHMENT 1-1.  Methodology for Estimating Exposures to Terrestrial Animals (mammals, birds, reptiles, amphibians and invertebrates) (DOCX)
  • ATTACHMENT 1-2. Aquatic Bin Assignments (XLSX)
  • ATTACHMENT 1-3. Explanation of Census Of Agriculture Table Generation (PDF)
  • ATTACHMENT 1-4. Usage Data Source Summary-Crosswalk (PDF)
  • ATTACHMENT 1-5. PVP Analysis (DOCX) 

Appendices

  • APPENDIX 1-1. List of Current Glyphosate Registrations (DOCX)
  • APPENDIX 1-2. Master Use Summary Table for Glyphosate (DOCX)
  • APPENDIX 1-3. Glyphosate Scenario Development (DOCX)
  • APPENDIX 1-4. Usage Data for Glyphosate (PDF)
  • APPENDIX 1-5. CDL to UDL Crosswalk for Glyphosate (DOCX)
  • APPENDIX 1-6. Use Site Footprint for Glyphosate (DOCX)
  • APPENDIX 1-7. Determination of Overlap of Likely Glyphosate Exposure Area and Listed Species Ranges and Critical Habitat for Species Located in the 48 Contiguous United States (ConUS) (DOCX)
  • APPENDIX 1-8. Determination of Overlap of Glyphosate Exposure Area and Species Ranges and Critical Habitat for Species Located Outside of the 48 Contiguous States (NL48) (DOCX)

Chapter 2: Final Glyphosate Effects Characterization (DOCX)

Attachments

  • ATTACHMENT 2-1. Effects Arrays (DOCX)
  • ATTACHMENT 2-2. Ecological Incidents (DOCX)

Appendices

  • APPENDIX 2-1. Data Used in the Data Array (XLSX)
  • APPENDIX 2-2. Accepted ECOTOX Database (XLSX)
  • APPENDIX 2-3. Open Literature Review for Glyphosate (DOCX)
  • APPENDIX 2-4. Glyphosate Studies Submitted to US EPA [from Office of Pesticide Programs Information Network (OPPIN)] (DOCX)
  • APPENDIX 2-5. Glyphosate Species Sensitivity Distribution Analysis for Aquatic Plants (DOCX)
  • APPENDIX 2-6. Glyphosate Species Sensitivity Distribution (SSD) Analysis for Terrestrial Plants (DOCX)

Chapter 3: Final Glyphosate Exposure Characterization (DOCX) 

Attachments

  • ATTACHMENT 3-1: Background Document: Aquatic Exposure Estimation for Endangered Species (DOCX)

Appendices

  • APPENDIX 3-1. Supporting Material For Aquatic Modeling (Glyphosate) (XLSX)
  • APPENDIX 3-2. Aquatic Modeling Runs (Glyphosate) (Please save this file prior to opening) (ZIP)

Chapter 4: Final Glyphosate Effects Determinations (DOCX)

Attachments

  • ATTACHMENT 4-1. Criteria for Strength of Evidence in LAA Determination (DOCX)

Appendices

  • APPENDIX 4-1. Species Effects Determination Tables (XLSX)
  • APPENDIX 4-2. Input Parameters for MAGtool (XLSX)
  • APPENDIX 4-3. Curve Number Scaling Factors (XLSX)
  • APPENDIX 4-4. Application Date Scaling Factors (XLSX)
  • APPENDIX 4-5. Analysis of Granular (DOCX)
  • APPENDIX 4-6. Analysis Method and Results for Downstream Monitoring (DOCX)
  • APPENDIX 4-7. Downstream Monitoring Analysis (XLSX)
  • APPENDIX 4-8. Additional Qualitative Species Analyses (DOCX)
  • APPENDIX 4-9. MAGtool and Weight of Evidence Output for Individual Species (ZIP)

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Last updated on October 1, 2024
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