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Clean Water Act Analytical Methods

  • Methods Update Rule 22

    The EPA proposed Methods Update Rule 22 to promulgate three new EPA methods into 40 CFR Part 136, and other actions.

    Learn more about the proposed rule
  • Routine Methods Update Rule 2

    On April 16, 2024, the EPA issued a final routine Methods Update Rule 2 under the Clean Water Act.

    View the Final Rule

The EPA publishes laboratory analytical methods (test procedures) that are used by industries and municipalities to analyze the chemical, physical and biological components of wastewater and other environmental samples required by the Clean Water Act.

Current and Recent Actions

  • Frequent Questions about PFAS Methods for NPDES Permits
  • Methods Update Rule 22 - Proposed Rule
  • Methods 1621 and 1633A for Adsorbable Organic Fluorine and PFAS
  • Routine Methods Update Rule 2 - 2023 Final Rule
  • Draft Method 1634 for 6PPD-q
  • Methods for 2,3,7,8-substituted PCDDs/PCDFs
  • PCB Congeners by Low-Resolution GC-MS - Method 1628
  • MDL Frequently Asked Questions

Approved CWA Methods

  • Chemical
  • Microbiological
  • Whole Effluent Toxicity
  • Radiochemical
  • Industry-Specific
  • Frequent Questions

Other CWA Methods

Optional methods for analyzing wastewater and biosolids (not approved under 40 CFR Part 136):
  • Chemical
  • Microbiological
    including MST Methods 1696 & 1697
  • Biosolids

Alternate Test Procedures

Development of modified EPA methods or new methods
  • ATP Overview
  • Protocol Documents
  • ATP Questions and Answers
  • How to Get Methods Approved

Regulations and Support Documents

  • Regulatory History for:
    • Methods Update Rules
    • Microbiological methods
    • Oil & grease
    • Cyanide
    • Dioxins & furans
    • Mercury and chromium-VI
  • Other methods support documents
  • Toxic and Priority Pollutant lists
  • Legal authority for methods

Related EPA Programs

  • NPDES permit program
  • Effluent Guidelines program
  • Other EPA method collections
    Including drinking water, air and solid waste methods
Contact Us to ask a question, provide feedback, or report a problem.
Last updated on April 23, 2025
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